Mouse Genome Engineering Using Designer Nucleases
نویسندگان
چکیده
منابع مشابه
Mouse Genome Engineering Using Designer Nucleases
Transgenic mice carrying site-specific genome modifications (knockout, knock-in) are of vital importance for dissecting complex biological systems as well as for modeling human diseases and testing therapeutic strategies. Recent advances in the use of designer nucleases such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly in...
متن کاملGenome engineering with targetable nucleases.
Current technology enables the production of highly specific genome modifications with excellent efficiency and specificity. Key to this capability are targetable DNA cleavage reagents and cellular DNA repair pathways. The break made by these reagents can produce localized sequence changes through inaccurate nonhomologous end joining (NHEJ), often leading to gene inactivation. Alternatively, us...
متن کاملGenome engineering with zinc-finger nucleases.
Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular DNA repair processes that lead to both targeted mutagenesis and targeted gene replacement at remarkably high frequencies. This article briefly reviews the history of ZFN development and summarizes applications that have been m...
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Transcription activator-like effector nucleases are readily targetable 'molecular scissors' for genome engineering applications. These artificial nucleases offer high specificity coupled with simplicity in design that results from the ability to serially chain transcription activator-like effector repeat arrays to target individual DNA bases. However, these benefits come at the cost of an appre...
متن کاملGenome engineering using DNA-binding proteins: zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs)
........................................................................................................... x CHAPTER 1. GENERAL INTRODUCTION ........................................................ 1 BACKGROUND ................................................................................................ 1 METHODS FOR ASSEMBLING TALENs. ...................................................... ...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2014
ISSN: 1940-087X
DOI: 10.3791/50930